Beilstein J. Org. Chem.2018,14, 397–406, doi:10.3762/bjoc.14.28
libraries can be prepared by a variety of methods, starting from the respective genelibrary. The challenge in genelibrary preparation is to achieve controlled total or partial randomization at any predefined number and position of codons of a given gene, in order to obtain a library with a maximum number
on soluble polymers, and their use as synthons in standard DNA synthesis.
Keywords: genelibrary; protein engineering; soluble support; synthesis; trinucleotide; Introduction
Protein engineering is a highly actual research area with a number of potential applications [1][2][3][4]. The construction
PCR are used for this purpose as well as recombinant methods like DNA shuffling and related strategies [6][7]. One of the major challenges in genelibrary production is to generate libraries with a high number of promising candidates to enhance the chance of selecting functional protein variants. The
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Graphical Abstract
Figure 1:
Preparation of fully protected trinucleotides in solution (A), on solid phase (B) and on soluble po...